Purification and partial characterization of a glycoprotein sexual inducer from Volvox carteri.
نویسندگان
چکیده
A method is described for the isolation and purification of the sexual inducer produced by male colonies of the colonial green alga Volvox carteri. The procedure yields a highly purified preparation of the inducer. The biologically active principal is demonstrated to be a glycoprotein with a molecular weight of 32,000.
منابع مشابه
The sexual inducer of Volvox carteri: purification, chemical characterization and identification of its gene.
The sexual inducer of Volvox carteri f. nagariensis is a glycoprotein and one of the most potent biological effector molecules known. It is synthesized by sperm cells and converts asexually growing males and females to the sexual pathway. Until now, large-scale production of the inducer was made impossible by an inherent biological ;switch' mechanism, the spontaneous self-induction of asexually...
متن کاملA Gender-Specific Retinoblastoma-Related Protein in Volvox carteri Implies a Role for the Retinoblastoma Protein Family in Sexual Development W OA
Here, we describe the cloning and characterization of RETINOBLASTOMA-RELATED PROTEIN1 (RBR1) from the green alga Volvox carteri. RBR1 expression increases substantially during embryogenesis and in response to the sex-inducer glycoprotein, but it decreases significantly under heat stress. While RBR1 is expressed in gonidia (asexual reproductive cells) and embryos, the largest proportion of RBR1 ...
متن کاملA gender-specific retinoblastoma-related protein in Volvox carteri implies a role for the retinoblastoma protein family in sexual development.
Here, we describe the cloning and characterization of RETINOBLASTOMA-RELATED PROTEIN1 (RBR1) from the green alga Volvox carteri. RBR1 expression increases substantially during embryogenesis and in response to the sex-inducer glycoprotein, but it decreases significantly under heat stress. While RBR1 is expressed in gonidia (asexual reproductive cells) and embryos, the largest proportion of RBR1 ...
متن کاملReporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri.
The multicellular alga Volvox is an attractive model for the study of developmental processes. With the recent report of successful transformation, regulated promoters as well as reporter genes working in this organism are now required. The Volvox genes encoding arylsulfatase and the extracellular glycoprotein ISG are strictly regulated. The former is transcribed only under conditions of sulfur...
متن کاملSexual Induction in Volvox Carteri
The study of the dependence of reactions on the concentration of various compounds is often of great value in determining reaction mechanisms . In chemical kinetics, it is often found that, according to van't Hoff's rule, the order of dependence of a reaction on the concentration of some compound involved in that reaction is equal to the number of molecules of the compound involved in each reac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 71 4 شماره
صفحات -
تاریخ انتشار 1974